Abstract
Several microwave-assisted digestion methods were tested at the Centro de Estudios Aplicados en Química laboratory in Quito, Ecuador, to determine the accuracy and performance efficiency of the mineralization process for the determination of total mercury in fish tissue by cold vapor atomic fluorescence spectrophotometry. The use of MARSEasyPrep high-pressure vessels, low amounts of reagents (1 cm3 HNO3, 1 cm3 H2O2, and 1 cm3 HClO4), an irradiation temperature of 210 ◦C, and 35 min of mineralization time resulted in accurate performance, with recoveries of certified reference material DORM-4 between 90.1% and 105.8%. This is better than the Association of Official Analytical Chemists 2015.01 method, which has a reported accuracy of 81%. The repeatability precision and intermediate precision were established at three concentration levels (0.167, 0.500, and 0.833 mg·kg−1) and expressed as the percentage of the relative standard deviation ranging from 1.5% to 3.0% and 1.7% to 4.2%, respectively. Further, the method was satisfactorily applied to analyze fortified samples of tilapia (Oreochromis niloticus), with recoveries ranging from 98.3% to 104.3%. The instrumental limits of detection and quantification were 0.118 μg·dm−3 and 0.394 μg·dm−3, respectively.
| Original language | Spanish (Ecuador) |
|---|---|
| Journal | Methods and Protocols |
| State | Published - 23 Jun 2020 |
| Externally published | Yes |
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NAVARRETE ZAMBRANO, HUGO. GUILLERMO. (2020). OPTIMIZATION OF A DIGESTION METHOD TO DETERMINE TOTAL MERCURY IN FISH TISSUE BY COLD VAPOR ATOMIC FLUORESCENCE SPECTROPHOTOMETRY. Methods and Protocols.
NAVARRETE ZAMBRANO, HUGO GUILLERMO. / OPTIMIZATION OF A DIGESTION METHOD TO DETERMINE TOTAL MERCURY IN FISH TISSUE BY COLD VAPOR ATOMIC FLUORESCENCE SPECTROPHOTOMETRY. In: Methods and Protocols. 2020.
@article{b58d10a1cb6d4db483afa2ad47a3b197,
title = "OPTIMIZATION OF A DIGESTION METHOD TO DETERMINE TOTAL MERCURY IN FISH TISSUE BY COLD VAPOR ATOMIC FLUORESCENCE SPECTROPHOTOMETRY",
abstract = "Several microwave-assisted digestion methods were tested at the Centro de Estudios Aplicados en Qu{\'i}mica laboratory in Quito, Ecuador, to determine the accuracy and performance efficiency of the mineralization process for the determination of total mercury in fish tissue by cold vapor atomic fluorescence spectrophotometry. The use of MARSEasyPrep high-pressure vessels, low amounts of reagents (1 cm3 HNO3, 1 cm3 H2O2, and 1 cm3 HClO4), an irradiation temperature of 210 ◦C, and 35 min of mineralization time resulted in accurate performance, with recoveries of certified reference material DORM-4 between 90.1% and 105.8%. This is better than the Association of Official Analytical Chemists 2015.01 method, which has a reported accuracy of 81%. The repeatability precision and intermediate precision were established at three concentration levels (0.167, 0.500, and 0.833 mg·kg−1) and expressed as the percentage of the relative standard deviation ranging from 1.5% to 3.0% and 1.7% to 4.2%, respectively. Further, the method was satisfactorily applied to analyze fortified samples of tilapia (Oreochromis niloticus), with recoveries ranging from 98.3% to 104.3%. The instrumental limits of detection and quantification were 0.118 μg·dm−3 and 0.394 μg·dm−3, respectively.",
author = "{NAVARRETE ZAMBRANO}, {HUGO GUILLERMO}",
year = "2020",
month = jun,
day = "23",
language = "Espa{\~n}ol (Ecuador)",
journal = "Methods and Protocols",
issn = "2409-9279",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
}
NAVARRETE ZAMBRANO, HUGOGUILLERMO 2020, 'OPTIMIZATION OF A DIGESTION METHOD TO DETERMINE TOTAL MERCURY IN FISH TISSUE BY COLD VAPOR ATOMIC FLUORESCENCE SPECTROPHOTOMETRY', Methods and Protocols.
OPTIMIZATION OF A DIGESTION METHOD TO DETERMINE TOTAL MERCURY IN FISH TISSUE BY COLD VAPOR ATOMIC FLUORESCENCE SPECTROPHOTOMETRY. / NAVARRETE ZAMBRANO, HUGO GUILLERMO.
In: Methods and Protocols, 23.06.2020.
Research output: Contribution to journal › Article › peer-review
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AU - NAVARRETE ZAMBRANO, HUGO GUILLERMO
PY - 2020/6/23
Y1 - 2020/6/23
N2 - Several microwave-assisted digestion methods were tested at the Centro de Estudios Aplicados en Química laboratory in Quito, Ecuador, to determine the accuracy and performance efficiency of the mineralization process for the determination of total mercury in fish tissue by cold vapor atomic fluorescence spectrophotometry. The use of MARSEasyPrep high-pressure vessels, low amounts of reagents (1 cm3 HNO3, 1 cm3 H2O2, and 1 cm3 HClO4), an irradiation temperature of 210 ◦C, and 35 min of mineralization time resulted in accurate performance, with recoveries of certified reference material DORM-4 between 90.1% and 105.8%. This is better than the Association of Official Analytical Chemists 2015.01 method, which has a reported accuracy of 81%. The repeatability precision and intermediate precision were established at three concentration levels (0.167, 0.500, and 0.833 mg·kg−1) and expressed as the percentage of the relative standard deviation ranging from 1.5% to 3.0% and 1.7% to 4.2%, respectively. Further, the method was satisfactorily applied to analyze fortified samples of tilapia (Oreochromis niloticus), with recoveries ranging from 98.3% to 104.3%. The instrumental limits of detection and quantification were 0.118 μg·dm−3 and 0.394 μg·dm−3, respectively.
AB - Several microwave-assisted digestion methods were tested at the Centro de Estudios Aplicados en Química laboratory in Quito, Ecuador, to determine the accuracy and performance efficiency of the mineralization process for the determination of total mercury in fish tissue by cold vapor atomic fluorescence spectrophotometry. The use of MARSEasyPrep high-pressure vessels, low amounts of reagents (1 cm3 HNO3, 1 cm3 H2O2, and 1 cm3 HClO4), an irradiation temperature of 210 ◦C, and 35 min of mineralization time resulted in accurate performance, with recoveries of certified reference material DORM-4 between 90.1% and 105.8%. This is better than the Association of Official Analytical Chemists 2015.01 method, which has a reported accuracy of 81%. The repeatability precision and intermediate precision were established at three concentration levels (0.167, 0.500, and 0.833 mg·kg−1) and expressed as the percentage of the relative standard deviation ranging from 1.5% to 3.0% and 1.7% to 4.2%, respectively. Further, the method was satisfactorily applied to analyze fortified samples of tilapia (Oreochromis niloticus), with recoveries ranging from 98.3% to 104.3%. The instrumental limits of detection and quantification were 0.118 μg·dm−3 and 0.394 μg·dm−3, respectively.
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M3 - Artículo
SN - 2409-9279
JO - Methods and Protocols
JF - Methods and Protocols
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NAVARRETE ZAMBRANO HUGOGUILLERMO. OPTIMIZATION OF A DIGESTION METHOD TO DETERMINE TOTAL MERCURY IN FISH TISSUE BY COLD VAPOR ATOMIC FLUORESCENCE SPECTROPHOTOMETRY. Methods and Protocols. 2020 Jun 23.
